Course Description
- This course covers the fundamental aspects of DNA and RNA structure, their function, and their interactions with proteins.
- The course includes DNA replication, DNA repair, transcription, synthesis of proteins, and the regulation of gene expression.
- The mechanisms of regulation of gene expression, the synthesis of proteins, protein-protein interaction domains, RNA translation, and Post-transcriptional mechanisms include mechanisms of RNA splicing, gene structure and function, and chromosome remodeling.
The course topics will be as follow:
What is the molecular biology, Concept of Genome and its organization, Packaging of DNA- nucleosome, chromatin, structure of chromosomes, Organelle genome (Mitochondria), DNA Replication, DNA Damage and Repair and Recombination, Transcription machinery in prokaryotes, Transcription in eukaryotes, Translation /Protein synthesis, Regulation of protein synthesis,Genomics and Proteomics, Techniques in Molecular Biology.
Practical content
This part of the course covers molecular techniques such as isolation and extraction of both DNA and RNA from animal and plant cells. The practical part also includes the following techniques; polymerase chain reaction, agarose and polyacrylamide gel electrophoresis and DNA sequencing.
Course Objectives & Outcomes
Objectives
- Understand the fundamental aspects of DNA and RNA structure, their function, and their interactions with proteins.
- Study DNA replication, DNA damage, DNA repair, and RNA transcription.
- Identify the transcriptional control mechanism.
- Involve with mechanisms RNA transitional and stability control.
- Understand posttranscriptional mechanisms.
- Understand protein synthesis.
- Explain mechanisms of gene expression and gene regulation.
Outcomes
At the end of this course, the student will be able to:
- The central dogma of biology.DNA Replication, DNA damage, and DNA repair.
- The transcription of RNA.
- Factors affecting RNA transcription such as RNA polymerases and chromatin remodeling.
- Gene regulation at the levels of transcription, RNA processing, transport, and stability.
- Mechanisms of RNA splicing, targeting RNA stability, and translational control.
- Genetic code and the synthesis of proteins.
- Regulator and repressor proteins.
- Gene structure and function.
- Molecular biology tools.
- Gene manipulation.
References
1. Pal J.K. &Ghaskadbi S.S. (2009) Fundamentals of Molecular Biology. Oxford, University press, India.
2. Molecular Biology of the cell (2014) Bruce Alberts, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter, 6th edition, Taylor and Francis group.
Course ID: GENTC 308
Credit hours | Theory | Practical | Laboratory | Lecture | Studio | Contact hours | Pre-requisite | 3 | 2 | 2 | 4 | BIOCH 308 - GENTC 304 |
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