Course Description
The course focuses on DNA, RNA and protein synthesis, Recombinant DNA Technology, gene expression in prokaryotes include Strain Isolation and screening, Fermentation and production of proteins from recombinant microorganism. It considers the mutagenesis and protein engineering and Industrial Application for example Production of antibodies in E. coli. And discuss Production of small biological molecules, Vaccines, Antibiotics, biopolymers. Define Bioremediations and Molecular Diagnostics
Practical content:
The course focuses on the following molecular techniques: Purification of mRNA, Reverse Transcription PCR, Gel Extract cDNA, Restriction Enzymes Digestion, Recombinant DNA, and Gene Expression. Apply Protein extraction, separation on (polyacrylamide gel), Electro blotting that include Blocking, Incubation, Detection and recognize 2D- gel electrophoresis
Course Objectives & Outcomes
Objectives
- Develop practical skills in applying molecular techniques.
- Acquire the intellectual ability and practical skills necessary to work productively in a commercial setting, institute, or university laboratory on different aspects of molecular microbiology and biotechnology.
- Become familiarized with the industrial applications of microorganism, such as enzymes, antibiotics production, bioremediation, and diagnostics.
Outcomes
At the end of this course, the student will be able to:
- Explain how microbiology is applied to manufacturing in the pharmaceuticals industry.
- Involvement in the production methods for pharmaceuticals of microbial origin such as antibiotics and vaccines.
- Involvement in the improvement of microorganisms.
- Understand the mutation of cloned genes and the protein engineering used to alter enzyme structure and function.
- Understand the fermentation processes.
References
1- Industrial Microbiology: An Introduction by Waites, Morgan, Rockey and Higton Blackwell Science (2001)
2- Brock biology of microorganism, 11 th edition, By Madigan,Michael and Martinko,John, (2005)
3- Elements of Chemical Reaction and Engineering, 4 thedition, by H. Scott Fogler Pearson Education Inc., (2006).
Course ID: BIOL 505
Credit hours | Theory | Practical | Laboratory | Lecture | Studio | Contact hours | Pre-requisite | 3 | 3 | 3 | 6 | - |
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