Course Description
This area (genetic engineering) involves research into the molecular basics of, transformation, and genetic activity and employing the techniques of high-precision genetic analysis with methods of analytical biochemistry for research, employ tools that enable the process of identification, insertion and transfer of genetic material in living organisms to improve the biological performance. Genetic engineering is now used in many applications, including forensic analysis, paternity disputes, medical diagnosis, genome mapping and sequencing, biotechnology industry and applications. The topics will be as follow: Isolation of DNA and RNA, quantification and gel electrophoresis, Nucleic acid labeling and hybridization, DNA sequencing, Restriction enzymes, Polymerase chain reaction, Genetic engineering and biotechnology.
Practical content
Isolation of DNA, Electrophoresis, Polymerase chain Reaction, PCR purification, Cloning (digestion, Digestion DNA insertion, ligation….), DNA, Sequencing.
Course Objectives & Outcomes
At the end of the course the student will be able to:
- Describe the general principles of gene organization and expression in both prokaryotic and eukaryotic organisms.
- Interpret the outcome of experiments that involve the use of recombinant DNA technology and other common gene analysis techniques.
- Explain various levels of gene regulation and protein function, including signal transduction and cell cycle control.
References
- An Introduction to Genetic Engineering. Third edition 2015, Desmond S. T. Nicholl. Cambridge University Press
Course ID: GENTC 502
| Credit hours | Theory | Practical | Laboratory | Lecture | Studio | Contact hours | Pre-requisite | 3 | 2 | 2 | 4 | GENTC 308 |
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