FF (19 -20)
Objectives
- Investigate experimentally and analyze the behavior and structure of macromolecules using spectroscopic techniques.
- Interact effectively in a team to communicate the experimental concepts, processes, and results effectively, both verbally and in written format.
Applications
Measurement of the electric conductivity of a Solution / Lambert- Beer′s law / Spectroscopy Characteristics of Chlorophylls and carotenoids / Absorption spectrum of hemoglobin/ Size Exclusion Gel Chromatography.
Tools and Devices
- Conductivity meter /Measuring cylinder 25 ml / beaker / Dropper /Distilled Water /Glass rood / Thermometer / Electrical heater.
NaCl ( Sodium Chloride ) solution :
CH3COONa (Sodium Acetate)
(Ammonium Sulfate) (NH4)2SO4
- Spectrophoto meter /Measuring cylinder 25 ml / beaker /Dropper / Distilled Water /Glass rood.
Solution : KMnO4 Putasium Permanganate
- Spectrophoto meter /Measuring cylinder 25 ml / beaker /Dropper /Distilled Water / Glass rood.
Solution:160 ml acetone +40ml distilled water /10gm plants leaf
- Spectrometer+3 tube / tubes holder / dropper (very small – small).
Solution: Prepared hemoglobin sample
- Prepacked gel filtration column/ tubes Collection / tubes holder/ Pipette/ column buffer (phosphate buffered saline) / Coomassie Brilliant Blue dye.
Sample : Mixture of (Vitamin B12 and Albumin)
